HIV Western Blot test is advised in individuals who are HIV positive by RAPID CARD or with following risk factors like:
The western blot is an antibody detection test. However, unlike the ELISA method, the viral proteins are separated first and immobilized. In subsequent steps, the binding of serum antibodies to specific HIV proteins is visualized.
HIV Western Blot is the confirmatory test for HIV infection.
POSITIVE - Positive is considered by the presence of at least two of the three bands representing the envelope bands (gp160, gp120, and gp41 of HIV-1 and gp36 of HIV-2) and gag gene p24.
NEGATIVE- Absence of all the bands except control band is considered as negative test.
Additional test (to be done only in HIV positive patients)
CD4 COUNT - provide prognostic information about health status of patient. (Link CD4 COUNT)
HIV viral load provide valuable prognostic information of the patient on treatment. (Link HIV viral load)
HIV Genotyping provide information regarding the resistance developed in patient of HIV hence it help clinician to provide better Anti-retroviral treatment to patient. (Link HIV Genotyping).